Composite

Part:BBa_K565004:Design

Designed by: Juan Ricardo Ortiz Meneses   Group: iGEM11_Valencia   (2011-09-18)


Microcin C51 production device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4082
    Illegal PstI site found at 759
    Illegal PstI site found at 1239
    Illegal PstI site found at 3088
    Illegal PstI site found at 4334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4082
    Illegal PstI site found at 759
    Illegal PstI site found at 1239
    Illegal PstI site found at 3088
    Illegal PstI site found at 4334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4082
    Illegal BglII site found at 1399
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4082
    Illegal PstI site found at 759
    Illegal PstI site found at 1239
    Illegal PstI site found at 3088
    Illegal PstI site found at 4334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4082
    Illegal PstI site found at 759
    Illegal PstI site found at 1239
    Illegal PstI site found at 3088
    Illegal PstI site found at 4334
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Please see K. Severinov et al. PROKARYOTIC ANTIMICROBIAL PEPTIDES, 2011, Part 5, 289-308, Class I Microcins: Their Structures, Activities and Mechanisms of Resistance., for info on this point.


Source

The sequence of the Escherichia coli pMccC51 plasmid mccABCDE genes have been deposited under the accession numbers AJ487788.2, in the EMBL/GenBank database. This part was central in our 2011 iGEM project: http://2011.igem.org/Team:Valencia


References